Working in the Automation Center of Excellence group in the Potency department. This role includes developing and running assays to assist Seagen and partner projects to enable future cancer treatments.

Validated protein structural models of antibody-antigen and antibody-peptide mask binding to better understand design of masked peptides for cancer therapeuticsDesigned, expressed, and tested mutant constructs using in vitro and in silico methodsUsed structural model to select a series of mutations to antibody and antigenCultured mammalian and bacterial cells and performed binding assays to affinity mature antibodiesComputationally modeled binding affinities and structures using… Show more Validated protein structural models of antibody-antigen and antibody-peptide mask binding to better understand design of masked peptides for cancer therapeuticsDesigned, expressed, and tested mutant constructs using in vitro and in silico methodsUsed structural model to select a series of mutations to antibody and antigenCultured mammalian and bacterial cells and performed binding assays to affinity mature antibodiesComputationally modeled binding affinities and structures using Schrodinger’s Bioluminate and PymolSkills in cell culture, transformation, transfection, ELIZA assays, Octet RED, Hamilton Microlab, and affinity purification, SnapGene, and Benchling Show less

Worked to understand precise assembly and function control of nanoscale protein structuresStudied in-solution properties of protein nanostructures in E. coli including re-solubilized membrane proteins and self-assembling 1D and 2D protein structuresExpressed, purified, and tested light-activated proton pump membrane proteinsDesigned, transformed, expressed, and purified novel protein constructsSkills in PCR, site directed mutagenesis, DNA and protein gel electrophoresis, western… Show more Worked to understand precise assembly and function control of nanoscale protein structuresStudied in-solution properties of protein nanostructures in E. coli including re-solubilized membrane proteins and self-assembling 1D and 2D protein structuresExpressed, purified, and tested light-activated proton pump membrane proteinsDesigned, transformed, expressed, and purified novel protein constructsSkills in PCR, site directed mutagenesis, DNA and protein gel electrophoresis, western blots, molecular cloning protocols, colorimetric assays, and protein expression studies Show less

-Developed data analysis for complex material properties and processes-Built skills in communication in teams and information searching in large databases-Presented analyses to others outside my group