Molly Likes Email & Phone Number

Boston, Massachusetts, United States

Greater Boston

Assistant coach for top level youth teams of all ages

Boston, Massachusetts, United States

Assistant coach for Emerson College Women's volleyball team.

Cambridge, Massachusetts, United States

- Performed experiments to determine best treatment concentrations, cell seeding density for treatment, and treatment times to enhance antigen surface presentation for Mass Spec analysis- Started an R&D project to implement 3D cell culture methods. Experiments focused on optimization of methods, ensuring uniform culture methods, and how to scale up spheroids to large cell counts for Mass Spec analysis. The goal of the project was to have more in vivo- like methods for R&D testing and optimization.- Trained others, within the group and across the company, on transfection, viral titration, transduction and selection, banking, growing cell lines up at a large scale, collecting small pellets for NGS and qPCR, treatment of large quantities of cells, harvesting Mass Spec pellets, RNA extraction, cDNA synthesis, and qPCR- Tested and implemented a new cDNA synthesis method to save time and money- Worked cross functionally with the automation team to troubleshoot our Hamilton for qPCRs- Performed large size data analysis on qPCR results- Presented cell line workflow and data in group and company meetings- Worked cross functionally across the company to implement Benchling ELNs, ELN templates, results schemeas, and inventories. Taught others how to effectively use and maintain the platform- Established insights pages on Benching using SQL to visually see the use of the platform- Shadowed cloning of plasmids (Gibson assembly, Sanger sequencing, clone selection, etc), ELISpots, Ribo-seq, and more- Accepted into Nature Medicine: “A Shared Neoantigen Vaccine Combined with Immune Checkpoint Blockage for Advanced Metastatic Solid Tumors: Phase 1 Trial Interim Results”

Cambridge, Massachusetts, United States

- Worked extensively with both suspension and adherent cancer cell lines, growing them up to high cell counts for Mass Spec.- Transfected plasmids into 293T cells to create a lentivirus stock for transduction into cell lines. These viruses ranged from HLA's to vaccine cassettes.- Transduced these viruses into cell lines of interest for cryopreservation and further testing. After selection, I would ensure proper expression within the cell line by qPCR.- Performed routine mycoplasma testing to ensure our cell lines remained contamination free.- Treated large quantities of cells to increase surface antigen presentation for Mass Spec analysis. Post treatment, large cell pellets were collected for Mass spec. Smaller pellets were collected for qPCR and NGS.- Completed these methods, transfection to cell pellet collection, for 10+ cells lines in one quarter- Performed data analysis on qPCR results- Presented cell culture workflow and results in group meetings-Assisted in the creation and optimization of Benchling ELN notebook and inventory

Boston, Massachusetts, United States

- Primary person receive blood and tissue samples from patients with Non-Small Cell Lung Cancer or Malignant Pleural Mesothelioma.- Isolated PBMCs and cryopreserved them- Process pleural effusions for cryopreservation- Dissociated fresh tissue sample from surgical cases for further analysis- Preformed 10X sequencing- Performed Seq-Well sequencing- Introductory cell culture

Cambridge, Massachusetts, United States

Assisted in research of medical devices and how we can build affordable versions for developing countries. Learned about biomedical engineering, how to properly do large-scale research on topics, and about medical access in developing countries.

Shepans Eye Institute, Boston

Assisted PhD student in writing a MatLab code to analyze microscopic cell images. The main purpose of the code was to determine the colocalization of different cell stains.

Wellesley, Massachusetts, United States

Melbourne, Florida, United States

- Shadowed and assisted PhD student with bio-chemical analysis on caged chemical compounds- Assisted with column chromatography set up and run, dry down of caged compounds for mass spec analysis, preparing samples for runs on Mass Spec machine, set- up of Mass Spec machine run, and data analysis of mass spec data

Norman, Oklahoma, United States

Coached girls competitive youth volleyball teams. Worked with the top teams for 12s, 13s, and 14s.

Bachelor Of Arts - Ba, Psychology, Pre-Med

Contributed To Wellesley College B.A.

Open Program, Pre-Nursing Studies

High School Diploma