Nicholas Sciascia, Ph.D. Email & Phone Number

Lexington, Massachusetts, United States

Cambridge, Cambridgeshire, GB

Designed and led a research project to explore role of BRD4 in acquired platinum resistance in ovarian cancer. Generated cell lines resistant to platinum agents and applied various molecular biology and sequencing techniques, such as high throughput fluorescence imaging, to assess the impact of BRD4 inhibition on kinetics of platinum adduct formation and.

Bethesda, MD, US

Led a multi-year, complex research project, examining how proteolytic degradation of topoisomerase-DNA complexes impacts genome integrity and DNA repair outcomes.Contributed to the development of a novel sequencing method (END-seq), as part of a large research team, to measure DNA double-strand breaks (DSBs) and DNA end-resection genome-wide at base-pair.

Bethesda, MD, US

Investigated the mechanisms by which the Fragile X Syndrome (FXS) causing gene FMR1 becomes abnormally silenced by epigenetic modifications.Tested candidate small molecule drugs targeting those epigenetic modifications in various FXS cells types (e.g. fibroblasts, iPSC, neuronal cell types) to determine if FMR1 reactivation can be achieved. Optimized.

Brentford, Middlesex, GB

Developed a small molecule-based small interfering RNA (siRNA) delivery vector, using the DNA Encoded Library Technology (DEL) previously established in the group. Collaborating as part of a larger team, I created cell-based assays to detect DEL molecule populations that were able to cross the cell membrane and enter the cytoplasm, and later recover them.

Cambridge, MA, US

Developed an ELISA assay to detect physiologic levels of the Lymphotoxin-alpha protein, which would be used to analyze samples from ongoing mouse studies, in pursuit of developing new antibody therapies to target autoimmune cells in Crohn’s and Graft vs. Host Disease. Using this ELISA assay, I assessed new antibodies that were synthesized by the protein.

Paris, France, FR

Optimized an ELISA assay to detect a target protein in mouse serum samples and assessed the success of the viral infection to create a normal gene product, as part of my group’s goal to identify novel ways to replace nonfunctional genes in living organisms using adeno-associated viruses as the delivery vectors. Assisted my colleagues with mice takedowns.

Doctor Of Philosophy - Phd, Molecular Medicine

Bachelor Of Science - Bs, Biology, General