Laboratory of Dr. John Fowlkes, Director of Barnstable Brown Kentucky Diabetes Center

• Molecular biology work in lab that researches Drosophila hormone receptor biology• Main project was constructing new subclones and mutations of several genes (retinoid X receptor analogs) involved in fruit fly development, combining Drosophila and mosquito DNA• Used quantitative RT-PCR to study Drosophila gene expression in response to hormone treatment• Managed laboratory supply orders, inventory, shipping and receiving• Position Eliminated

• Produced recombinant proteins involved in HIV pathogenesis• In charge of bioreactor-based protein production and purification• Distributed protein to interested parties on campus, in the US and abroad • Worked in collaboration with materials engineering laboratory personnel to develop novel technique for protein purification• Oversaw all aspects of the operation, including developing and refining techniques• Managed laboratory with almost complete independence and autonomy after 2002 • Position Eliminated

• Studied autoimmune uveitis in mice using various molecular biological and immunological techniques.• Produced recombinant proteins for immunological research• Managed laboratory and acted as liaison to other laboratories, teaching personnel our research methods• Position Eliminated

• Studied plant gene responses to pathogens in Dr. Joe Chappell's lab• Studied regulation of genes in the isoprenoid pathway

• Research on lymphocyte class switching and apoptosis in the lab of Dr. Mark Boothby• Used a variety of molecular biological and immunological techniques• Managed laboratory, including mouse colony