Prachi Desai Email and Phone Number

1. Development of systemic targeted radiopharmaceuticals2. Maintaining collaborations and co-ordination of pre-clinical projects with different CROs3. Establishment of lab-based protocols to further advance pre-clinical projects4. Presentation of project results to the management team

Jul 2022 - Dec 2022 (On site)1) Optimization of formulation development of nanogels for in vivo therapy studies 2) Evaluating the receptor expression of the cells after treatment with different doses 3) In-vitro evaluation of activated macrophage surface markers under different conditions4) Writing scientific articlesDec 2022 - Present (Remote)1) Co-ordination of review article for clusters of excellence germany 2) Literature review of different delivery systems (oral, local, intravenous)3) Conceptualization, Writing, editing, proof-reading of the article

Potential of theranostic nanogels for endogenous radiotherapy 1) Pre-polymer functionalization, formulation development, and characterization of nanogels2) Radiolabeling of peptides and nanogels (Gallium-68 and Lutetium-177) and its in-vitro evaluation3) In-vivo evaluation of radiolabeled peptides and nanogels4) Demonstrated different strategies to improve the blood circulation half-life, tumor accumulation, and therapeutic potential of radiolabeled nanogels and peptidesManagement experience 1) Planning, execution, and analysis of different projects2) Assisted in writing proposals (DFG and animal proposals)3) Manage collaboration projects & communicate results with the collaboration partners4) Presentation of research as poster presentation or oral talks

1) Formulation development, analysis and characterization of complex injectables (microparticles & liposomes)2) Preparation of study files and scientific reporting of data/results 3) Knowledge of process-based risk assessment and familiarity with Design of Experiment (DoE)4) Involvement in Pilot plant- scale up activities5) Responsible for writing the stability protocol for Diluent and execution of Diluent stability studies6) Gained familiar knowledge on working according to GMP regulations, GLP7) Experienced in In line high shear mixer, solvent emulsion/evaporation, extrusion, TFF, homogenizer8) Collaboration with teams from different disciplines

1) Developed microsphere formulations via the membrane emulsification technique for the extended release of small molecules and proteins2) Analytical characterization & data analysis 3) Optimized the formulations according to the target release4) Presentation of results in the form of oral presentation

1) Distillation of the pluronic polymer for efficient polymer modification2) Synthesis of modified Pluronic polymer with carbonyldiimidazole (CDI) and subsequent coupling of ethylene diamine (EDA) to the both ends of the polymer block3) Characterization of the modified polymer with FT-IR

1) Planned, managed, and executed the formulation & process development of protein loaded polymeric nanoparticles 2) Developed analytical methods to characterize the formulations3) Performed in-vitro studies to investigate their cytotoxicity, internalization and hemo-compatibility4) Collaborated to develop a bioactive silicate Nanocomposite Hydrogel that induced osteogenic differentiation in human mesenchymal stem cells without using any growth factors5) Wrote a book chapter on Nanomaterials for Dental Tissue Engineering which discusses the applications of different materials in dentistry and its advantages6) Supervised undergraduate student projects

1) Synthesis of drug-cholesterol reaction2) Checking the reaction completion using Thin layer chromatography (1% methanol)3) Purification of the drug-cholesterol reaction & drug loaded liposomes using column chromatography4) Formulation & analytical characterization of magnetic liposomes via the thin film hydration method to achieve a therapeutic effect against lung cancer 5) Evaluated the toxicity and effectiveness of the formulation containing anti-cancer drugs on cancer cell lines (A549 and A4T1) using the MTS assay

Creating repository of Mycobacterium tuberculosis gene clones1) Isolation of genomic DNA from Mycobacterium tuberculosis H37Rv strain followed by gel electrophoresis2) Amplification of the specific drug target gene by PCR3) Ligation and cloning of amplified genes using TOPO TA cloning kit to yield recombinant plasmid4) Transformation of recombinant plasmid in DH5α Escherichia coli cells5) Selection of colonies and isolation of recombinant DNA followed by gel electrophoresis to confirm the reconstructionStandardization of growth conditions for production of antibacterial secondary metabolites from Oceanobacillus iheyensis DSOA1) Standardized the growth medium by varying several components in the culture medium 2) Maintenance of Oceanobacillus iheyensis DSOA in glycerol stock3) Culturing of Oceanobacillus iheyensis DSOA & Biomass estimation4) Extraction of secondary metabolites from chloroform5) Antimicrobial assays of chloroform extract by disc diffusion method6) Estimation & extraction of proteins using Lowry method and SDS-PAGE