Molecular Research Assistant
La Jolla, Ca, Us
At Professor Randolph Hampton’s Lab at UCSD, I participated in the research of cytoplasmic quality-control of misfolded-protein degradation in yeast. Under the guidance of a Ph.D. student, I performed biotechnological techniques such as cloning, PCR, making competent cells, and transforming with plasmids. I tested bacterial and yeast cells for variances in proliferation and for their responses to protein degradation once injected with chaperone-assisted ubiquitination. As part of the collaboration, I attended the labs' weekly group meetings and presented my own participation in assisting with development of FLAG tag stGND 1 gene for studying of protein degradation and studying chaperone proteins in proteolysis. SKILLS: Micropipettes, Making a pH buffer; Quantitative Measurements, centrifugation, ammonium sulfate precipitations; prepare size exclusion column, Affinity chromatography purification, Size exclusion chromatography purification, gel electrophoresis, Western blot, SDS-PAGE and electroblotting, Immunodetection, ELISA, restriction enzyme digest, Make competent cells and transform with plasmid, Purification and analysis of fluorescent proteins, SDS-PAGE of fluorescent proteins