Relevant Coursework / Lab Experience:- Principles and Applications of Bioseparations -Covered recovery and purification of biomolecules, especially recombinent proteins. Skills gained in gel electrophoresis, UV/Vis and fluorescence spectroscopy measurement, and SDS-PAGE methods. Developed familiarity and understanding of capillary electrophoresis and chromatographic separation methods: anion exchange, cation exchange, hydrophobic interaction, and size exclusion chromatography.- Introduction to Downstream Process Development -Covered unit operations and the parameters that control protein isolation and purification of a recombinant protein produced by E. coli. Examined the following operations: centrifugation, homogenization, chromatography, and filtration: tangential, UF/DF. Gained skills in HPLC column packing, reading chromatograms, and developed further understanding of IEC, HIC, and SEC methods.- cGMP Downstream Operations -Covered application at production scale and evaluation of the inherent issues resulting from the integration of recovery and purification unit operations, scale-up/production issues, and current Good Manufacturing Practice (cGMP) compliance. Pilot-scale laboratory experiences in cell removal, cell disruption, purification, and manufacturing quality systems that simulate downstream commercial cGMP facility. Performed manufacturing-scale column packing, followed SOPs, and maintained batch records while complying with cGMP practice.Specialties: Qualified by approximately a full year of biotechnology experience in process optimization and SOP documentation. Additional hands-on experience in control and scale-up of downstream production-scale bioprocesses in a cGMP environment. Expertise in column chromatography, HPLC, bioreactor cell culture techniques, and molecular techniques.