Steven Lim Email & Phone Number

London, England, United Kingdom

London, England, United Kingdom

Melbourne, Victoria, Australia

Charterhouse Square, London

Operation and maintenance of the core facility's cytometry equipment. Routine acquisition and analysis of samples on a number of BD cell sorters and analysers, an Imagestream X MkII and CyTOF2. Engaging with instrument users and assisting with experimental design and analysis.

Cardiff, United Kingdom

 Participation in early stages of a cell therapy clinical trial, requiring my expertise in flow cytometry and PBMC isolation Effective use of the internal quality management system Q-Pulse Directly handled the resolution of user-related instrument problems and hardware issues Quickly adapted to a new overseas working environment Maintenance and operation of the institution’s flow cytometers.o BD Biosciences: FACS Canto II, LSR Fortessa SORP, Aria IIIo Beckman Coulter: CyAN ADP Operated within the framework of CBS’s ISO 9001:2008 certification, ensuring a consistently high level of work Managing user booking requests and providing instruction in data analysis and instrument training Confident with polychromatic flow cytometry panel design

• Operated and maintained a number of cytometers during my supervision of the Monash Medical Centre core flow facilityo Beckman Coulter: MoFlo XDP, Navios, Legacy sorter and analysero Becton Dickinson: FACS Canto II • Preparation of the core facility’s quarterly business statements and overseeing the scheduling of user requests for reservations of instruments• Conducted ongoing daily QA/QC, maintenance and instrument troubleshooting• Routine sorting of biological samples• Training and assisting users with data analysis and experimental design

• Confident in handling human blood; including isolation, culture and cryopreservation of peripheral blood mononuclear cells• Mammalian tissue and microbial culture skills of a high order, with very good aseptic technique• Familiar with molecular biology techniques; including DNA/RNA extraction, genotyping, immunoblot, ELISA and cell signalling• General lab upkeep, including purchasing and preparation of consumables and reagents

Bachelor Of Science (Honours), Microbiology, General

Honours student working under the supervision of Dr Terry Kwok and Dr Rebecca Gorrell. Research thesis titled "Functional characterisation.

Bachelor Of Science (Bs), Microbiology And Immunology