Multi product manufacturing suit of mAbs for commercial market and clinical studies. *Downstream operations of NGF mAb drug substance from eukaryotic expression system. (LIBRELA, a product of Zoetis; Approved by US FDA)*Downstream Operations of HIV mAb drug substance from eukaryotic expression system. (A product of IAVI organization) *Downstream Operations of FmAb drug substance from eukaryotic expression system. (A product of BICARA)*Downstream Operations of anti CD20 mab drug substance from eukaryotic expression system. (A product of Zenoaq)

Viral Culture and Downstream Operations of Japanese Encephalitis Drug Substance. (JEEV - WHO Pre-qualified.)

Downstream Manufacturing Operations of Ad26.COV2.S Drug Substance (JCOVDEN a product of Janssen Pharmaceuticals; Approved by WHO, US-FDA and EMA regulatory agencies).*Operation of single use AKTA ready XL chromatography system (Make- Cytiva), single use multi channel TFF system (Make-Sartorius) and single use filtration and filling system for drug substance (Make-Parker).*QMS Elements (Change control annexure preparation for all introduction & changes, Initial investigation write-up for the deviation, CAPA implementation and evaluating the effectiveness.)

Brown field project - Microbial suite Downstream Manufacturing Operations of Peg-filgrastim Drug Substance from Prokaryotic expression system. (FULPHILA a product of Biocon Biologics; Approved by US-FDA, EMA, Health Canada and TGA regulatory agencies.) * Handling and operating knowledge of AKTA Process powered by UNICORN 7.0 & UNICORN 7.5, Chromatography columns (Axichrom, Chromflow, Quickscale, PALL resolute & BPG columns), Automated process vessels powered by Delta V & WinCC operating systems, TFF System (Make-Millipore), *Packing and Evaluation of Axichrom, Quickscale and BPG columns. *QMS Elements (Investigation report preparation, Change Management, CAPA Periodic SOP revisions and Batch manufacturing record revision as a part of action implement).*Protocol preparation and execution of periodic performance verification of equipment.

Green field project - Microbial suite Downstream Manufacturing Operations of Filgrastim and Pegylated Filgrastim Drug Substance from Prokaryotic Expression System. (Approved by EMA, Health Canada and CDSCO regulatory agencies)*Overall handling knowledge of IQ, OQ, PQ, periodic validation, and periodic calibration related to downstream processing equipment.*Handling and Operating knowledge of 80m2 TFF System (Make-Sartorius), AKTA Process, Automated process & buffer vessels and Chromatography columns (Chromoflow, Quickscale, and Axichrom columns).*Area compliance as per cGMP of EU Regulatory Bodies.

Bulk DS Manufacturing of Recombinant Hepatitis B surface antigen from Prokaryotic expression system. *Purification operations of HEP-B drug substance with Hyflo Supercel, Plate and frame filtration, TFF Operation, Chromatography techniques (Ion Exchange, Gel Filtration, Affinity and SE-HPLC).*CIP and SIP of process and buffer vessels for downstream operations.*Column packing (BPG column).*Routine and periodic calibration of Instruments. *Compliance of the area and equipment as per cGMP norms.

*Target molecules production(TNF alpha, hVEGF) @ laboratory scale. *Molecular Cloning (rhHu Proteins – Microbial Culture(E.Coli)), *Bio-Analytical Techniques (PCR, Gel Electrophoresis, SDS PAGE, Western Blotting & ELISA),*Upstream processing-Lab Scale(Shake Flask Method), *Media Optimization, Clone Optimization & Protein Expression studies*Downstream Processing (Gel Filtration, Ion Exchange & Affinity Chromatography ),*Bio-polymer research studies (Chitin & Chitosan developement),