Wayne Ernst Email & Phone Number

Cleveland, Oh, Us

Analysis of next-gen sequencing data for clinical samples utilizing the ThyroSeq test, including gene variant evaluation/curation and report preparation.

Variant categorization and interpretation of clinical next-gen sequencing data from targeted solid tumor and whole exome/transcriptome panels.

● Variant and gene interpretation of clinical NGS data related to cancer genomics.● Collaborate with fellows and faculty to prepare results and reports for clinical molecular pathology cases.● Training co-workers in case interpretation and clinical NGS workflow.● Design and validation of targeted NGS panels.● Generation and sequencing of DNA/RNA libraries on Ion Torrent NGS platforms.● Manual and automated NGS library preparation (QIAgility and Tecan).

Pittsburgh, Pa

Characterization of CFTR regulation in response to liped homeostasisMolecular & cell biology techniques include:● Protein isolation for Western blotting, coimmunoprecipitations, and pulldown assays.● Confocal immunofluorescence microscopy.● Site directed mutagenesis and epitope tagging of plasmids.● Human and bacterial cell culture.● Transient transfections

Doctor Of Philosophy - Phd, Molecular And Human Genetics

Characterization of T-type calcium channels in absence epilepsy. Molecular biology techniques include: ● Reverse transcription.

Bachelor Of Science - Bs, Genetics