Yi-Kai Su Email & Phone Number

Toronto, Ontario, Canada

Mississauga, Ontario, Canada

➢ Mammalian cell culture (CHO and HEK293) for upstream processes from shake flasks to 200 L. Lead the GMP upstream process on the floor for biologics production. Prepare and review GMP documents such as MBPRs, SOPs, etc. ➢ Technology transfer & process development. SME to assist with engineering/IT/validation for upstream equipment qualification.

Mississauga, Ontario

➢ Construct a predict KLa model from 3 L Mobius SUB using both RSM approach and nonlinear regression model (with P/V and Vg). ➢ Compare MODD and R: using RSM to investigate the effect of agitation and air input to the KLa in a 3 L bioreactor➢ Mammalian cell culture (CHO and HEK293) for upstream processes from shake flasks to 200 L. GMP campaign experience➢ Technology transfer & ups process development. SME to assist engineering/IT/validation for upstream equipment qualification.

➢ Development a high throughput screening assay to evaluate the efficacy of disinfectant formulation (QUAT-based and H2O2-based). ➢ Formulation development by applying DoE approaches (continuing the postdoc project) using R and JMP Pro.

London, Canada Area

➢ Applying R and JMP Pro for statistical analyses and DoE approach to disinfectants development (industrial project).➢ Using scanning electron microscope to characterize nanoparticles (clean room).➢ Genome sequence, assembly, and genes annotation of a Spathaspora passalidarum mutant YK208-E11 derived from evolutionary engineering (published on FEMS Yeast in 2018). ➢ Akali-pretreatment for corn stover and corn cob.➢ Basic microbiology and molecular biology assay, including spot growth assay, selection/screening plating, ATP assay, tetrazolium assay, fluorescent assay, and genome extraction.➢ Syngas fermentation – using strict anaerobes converting syngas to liquid fuels.➢ Biomass fermentation – converting biomass into fuels using yeasts.➢ Biomass composition analyses – analyzing corn stover samples from research institutes or government agencies (BIC, OMAFRA, AAFC, etc).

Madison, Wisonsin

➢ Lead and perform fermentation trials to assist R&D group and help Xylome to obtain NSF SBIR II grant. ➢ Apply DoE approach to develop a bioprocess, including medium formulation and SOP development for bio-oil production. ➢ Rebuild an Agilent 1100 bought from Amgen auction. ➢ Develop a high throughput assay for Nile Red lipid screening.➢ Develop a high throughput protocol of measure reducing sugars using 3,5-Dinitrosalicylic acid (DNS).➢ Evaluate the fermentability the sugarcane bagasse to ethanol (GeoSynFuels, LLC).➢ Investigate the cause of stall fermentation in a lactose fermentation plant.

Biological Systems Engineering And Great Lakes Bioenergy Research Center

➢ Adaptation and evolutionary engineering using pH-autostat and continuous bioreactors to select native yeast mutant strain capable of anaerobic growth with xylose as sole substrate.➢ High throughput screening to identify potential mutant strain with increase fermentation ability. ➢ Use lipogenic yeast for Biodiesel production(published on Bioresource Technolgy in 2015)➢ Investigate the possibility of using Spathaspora passlidarum for lignocellulosic conversion: − Strain comparison with other native xylose-fermenting yeast (published on AEM in 2012).− Effects of aerations and temperatures on cell growth, ethanol and polyol accumulation (published on Biotechnology and Bioengineering in 2014).− Using DoE approach to investigate the effects of combining difference raw nitrogen materials for fermentation ability. ➢ Qualitative and quantitative analyses of sugars, organic acids, polyols and ethanol by HPLC and GC.➢ Perform HPLC and GC maintenance/repair.

Department Of Bacteriology

Teach Micro304 “Biology of Microorganisms Laboratory”, an entry-level microbiology laboratory course for biology/microbiology major undergraduates. The job duty includes:1) Introducing lab course concept and presenting a 30-40 min lecture for the lab activity2) Assisting students to understand the lab materials, including in-class or out-class office hours/discussion section.3) Grading lab report, lab note and exams4) Designing the exam paper 5) Evaluating students’ performance base on lab reports, lab notes, exams, in-class activities, and attendance

Madison, Wisconsin

My job duty was to operate four 2-L fermentors on a weekly basis and performed data analyses in an interdisciplinary research team involved microbiologist, molecular biologist, and biochemists. I was also responsible for sugar and ethanol analyses by HPLC and HPLC maintenance/repair

Department Of Civil And Environmental Engineering

1) Photo-bioreactor operation and photoheterotrophic biohydrogen production; gas composition and organic acids analyses (GC), PHB and PHV analyses (GC), general wastewater analyses and GC maintenance. 2) Minimizing solid waste by sequential aerobic/anaerobic operation: Gas composition analyses (GC), organic acid analyses (HPLC), GC and HPLC maintenance. 3) Data compiling and analyses. 4) Perform modeling bacterial growth using excel-solver.

Doctor Of Philosophy - Phd, Biological Systems Engineering

lignocellulose bioconversion, yeast microbiology, and bioreactor operations

Master Of Science (M.S.), Civil And Environmental Engineering

Bachelor Of Science - Bs, Environmental/Environmental Health Engineering