Daniel Miller Email & Phone Number

Providing technical and troubleshooting support to current and potential customers of MaxCyte’s Scalable Transfection Systems. Currently working with customers in the Central US.

Greater St. Louis

Scientist – Suspension Culture Specialist M6P Therapeutics (Venture capital backed startup), St. Louis, MOHigh throughput small scale protein production:• Transient expression of lysosomal enzymes proteins for vector optimization.• Executed independent projects producing recombinant enzymes in mammalian cultures (CHO)• Exceeded on CQAs and delivery deadlines.Small scale protein production (10mL) in mini bioreactors.• Transient Large Scale Protein production• Transient expression of lysosomal enzymes proteins for preliminary assay development.• Lead project presentations and project update meetings.• Participated in enzyme activity assay development• Development of a stable pool platform• Developed protocol for stable bulk pool production of key lysosomal enzymes to reduce costof transient transfection/productions• Completed bulk pools for enzymes using chemical transfections.• Began project to utilize electroporation for bulk pool generation.

Greater St. Louis

Scientist – Cell Sciences and Development Dec 2017- June 2020SAFC MilliporeSigma, St. Louis, MOo CLE customer service project:• Executed a mostly independent project with support from senior staff for customercommunications.• Exceeded all CQAs and delivered double the requested cell lines.o CLD customer service project:• Developed a novel NIST cell line for the National Institute of Standards and Technology.• Lead customer presentations and project update meetings (with prep help).• Production range of unoptimized clones 2-3 g/L.• Peak titer in experimental conditions 6g/L.

Greater St. Louis Area

Associate Scientist – Cell Sciences and Development May 2015- Dec 2017SAFC MilliporeSigma, St. Louis, MOMy primary research involved using CRISPR/Cas9 and ZFN technologies to genetically engineer celllines to resist viral contamination, creating a safer biomanufacturing cell line (Safety CHO, laterrenamed Centinel)CLE Projects• Personally generated 27 targeted knockout cell lines that were tested with MVM viralchallenge assays.• Developed parallel knockout and triple knockout protocols utilizing labeled CRISPR and FACS• Utilized CRISPR/Cas9 and ZFN technologies for cell line engineering purposes.• Mammalian cell culture working with suspension CHO cells (CHOZN platform)• Standard transfection of plasmids and mRNA using electroporation• Working knowledge of Geneious Sequencing analysis software• Bacterial Transformation, TOPO vector cloning, and Maxiprep plasmid isolation

Greater St. Louis Area

At Patheon I worked as part of a team to develop downstream processes for protein purification and primarily worked on two different projects. One involving the purification of a monoclonal antibody using affinity chromatography to support upstream development of new media formulations. The second was a full development project for the purification of an enzyme to be used as an ultra-orphan drug.

Rolla Missouri

Dr. Katie Shannon's lab works with budding yeast to investigate the cell cycle regulation of cytokinesis and the actin cytoskeleton. My project dealt with Iqg1, a scaffolding protein that binds F-actin, and how it's regulation by the CDK Cdc28 and it's phosphatase Cdc14 affects the timing of actin ring formation and cytokinesis.

Master'S Degree, Cellular And Molecular Biology, 3.8

Activities and Societies: Phi Sigma honors society, iGEM, Undergraduate Research.

Bachelor Of Science (Bs), Cell/Cellular And Molecular Biology, 3.36

Activities and Societies: Phi Sigma, iGEM, Undergraduate Research