David Austin Email & Phone Number

-Optimized genetic manipulation of 3D organoids, enhancing transfection and gene editing efficiency to facilitate functional studies in complex tissue models.-Designed and implemented automated 3D organoid cell passaging systems, optimizing workflows and increasing reproducibility for advanced tissue models.-Specialized in 3D organoid cell culture, establishing protocols for the growth, maintenance, and analysis of complex organoid systems for preclinical applications.-Identified… Show more -Optimized genetic manipulation of 3D organoids, enhancing transfection and gene editing efficiency to facilitate functional studies in complex tissue models.-Designed and implemented automated 3D organoid cell passaging systems, optimizing workflows and increasing reproducibility for advanced tissue models.-Specialized in 3D organoid cell culture, establishing protocols for the growth, maintenance, and analysis of complex organoid systems for preclinical applications.-Identified and developed innovative cell culture technologies to meet evolving needs in advanced cell culture systems for the Life Science community.-Advanced and implemented preclinical models focusing on liver, small intestine, kidney, and brain tissues to enhance drug safety assessment and target biology understanding.-Collaborated with cross-functional teams and internal partners in an international, cross-sectoral project setting, providing expertise in advanced tissue culture models.-Specialized in iPSC-derived organoid development, working closely with Merck Healthcare colleagues to enhance organoid systems.-Extensive cell culture expertise and academic networking, leveraging strong connections within the academic sector to support project needs and laboratory advancements. Show less

Part-time 10+ hours-Supervised lecture and laboratory Bio3 evening classes. Taught the following topics: basic molecular and cellular biology, genetics, the anatomy and physiology of plants, animals, and humans, the diversity of life, evolution, and ecology -Communicated current environmental issues and new developments in the biological sciences -Laboratory experiences are integrated with lectures and stress scientific methodology and thinking

Full-time 40+ hours-Led CRISPR/Cas9 initiatives through cell line development that increased crude and purified Adeno-Associated Virus (AAV) full-to-empty ratios in cellular extract -Led Flow Cytometry assay development to test for AAV mammalian infectivity and mammalian transfection buffer assay development-Led mammalian cell culture laboratory to maintain cell lines for flow cytometry assays, mammalian transfection assay development, and AAV buffer development-Oversaw daily… Show more Full-time 40+ hours-Led CRISPR/Cas9 initiatives through cell line development that increased crude and purified Adeno-Associated Virus (AAV) full-to-empty ratios in cellular extract -Led Flow Cytometry assay development to test for AAV mammalian infectivity and mammalian transfection buffer assay development-Led mammalian cell culture laboratory to maintain cell lines for flow cytometry assays, mammalian transfection assay development, and AAV buffer development-Oversaw daily operations of the flow cytometry and cell culture laboratories, including maintenance, inventory, and user communications-Managed upstream and downstream AAV production and testing-ddPCR assay development -Generated 15+ unique cell lines through gene manipulation-Prepared white papers and facilitated protocol transfers to contract research organization-Managed two research associates-Met and/or exceeded all project goals and performance reviews-Played a crucial role in the launch of over 60 products dealing with AAV process development buffer and ddPCR assay buffers -Kept abreast of current biomedical science in the literature related to the functions of the current or future project. Recommended appropriate course of action to Research and Development Director, as deemed necessary Show less

Full-time 40+ hours-Developed, optimized, and implemented CRISPR/Cas9 technology for gene manipulation-Designed and optimized guide-RNA for CRISPR, maintained CRISPR libraries, proficient in next-generation sequencing for CRISPR pooled libraries-Led all lentiviral and protein production for core laboratory users and/or projects -Trained in primary cell immortalization, high throughput screening, and automated liquid handling -Proficient in recombinant DNA and bacterial… Show more Full-time 40+ hours-Developed, optimized, and implemented CRISPR/Cas9 technology for gene manipulation-Designed and optimized guide-RNA for CRISPR, maintained CRISPR libraries, proficient in next-generation sequencing for CRISPR pooled libraries-Led all lentiviral and protein production for core laboratory users and/or projects -Trained in primary cell immortalization, high throughput screening, and automated liquid handling -Proficient in recombinant DNA and bacterial cloning and compound library management-Managed Flow Cytometry instrument (BD Aira Fusion III) data acquisition, data analysis, instrument maintenance, and user communication-Managed high-throughput screening data acquisition and transfer-Generated over 300 cell lines with the use of CRISPR and lentiviral technology-Drafted, refined, and implemented standard operating procedures (SOPs)-Consistently met and/or exceeded all project goals and performance reviews-Kept abreast of current biomedical science in the literature related to the functions of the current or future project-Published three scientific papers with authorship Show less

High Throughput Screening (HTS), Automated Liquid Handling,Large Particle Flow Cytometry, Compound library management, Student assistant training and supervision, User / Client / Assay based specialized service, SOP creation, refinement, and implementation,Data acquisition, management, and analysis

Los Angeles, Ca

Full-time 40+ hours Developed novel drug targets in Triple Negative Breast Cancer (TNBC) health disparities  Created novel TNBC cell lines using RNAi, CRISPR, and lentiviral transduction methods  Supervised and managed flow cytometry instruments (AttuneNxT flow cytometer, BD FACSMelody cell sorter) and Leica confocal microscopy operations Published two first-author scientific papers

Doctor Of Philosophy (Ph.D.), Cancer Biology

Master Of Science - Ms, Biology

Bachelor Of Science - Bs, Biology