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I’m seeking Principal or Staff Scientist role at biotechnology and pharmaceutical companies and am the perfect fit for this role because I have the following skills: experimental design, assay development, project management, research and development, microscopy, flow cytometry, SOP generation, cross-functional collaboration and problem solving.I have the ability to work autonomously contributing on ddPCR protocols, flow cytometry assays, AAV/lentiviral process development, and cell culture maintenance, as evidenced by the creation and implementation of 6 standard operating procedures (SOPs) that enhanced laboratory efficiency and consistency. I have also spearheaded Flow Cytometry assay development for AAV mammalian infectivity testing and mammalian transfection buffer assay development, and generating over 150 unique cell lines through gene manipulation.I have experience reviewing reporting and analysis plans consistently meeting and exceeding all project goals and performance reviews, while supervising and managing flow cytometry instruments and Leica confocal microscopy operations, while working with a team of 3 members.-Flexible and versatile researcher and highly skilled scientist with over eight years of experience in cellular, molecular, genetic biology, specializing in CRISPR/Cas9 technology, as evidenced by 10 successful experiments per week related to gene therapy and vaccine research.-Ability to manage projects and priorities in diverse areas, with experience in flow cytometry, synthetic biology, cell line development, mammalian cell culture, and assay development, as evidenced by successfully launching over 60 products related to AAV process development buffer and ddPCR assay buffers.-Strong communication skills facilitating protocol transfers to Contract Research Organizations (CROs) and leading internal departmental meetings, resulting in 10 impactful presentations that enhanced collaboration and alignment across teams.I want to serve an organization that prizes reliability, autonomy and challege among its employees. I’m excited to be on a team built around collaboration, work ethic and happiness. I know I will thrive at a company where loyalty is valued, as well as leadership and stability. I want to be a part of an organization that infuses sustainability into their brand, culture and market and who I can help make a difference in the world.
Milliporesigma
View- Website:
- sigmaaldrich.com
- Employees:
- 4902
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Scientist IiiMilliporesigma Oct 2024 - Present-Optimized genetic manipulation of 3D organoids, enhancing transfection and gene editing efficiency to facilitate functional studies in complex tissue models.-Designed and implemented automated 3D organoid cell passaging systems, optimizing workflows and increasing reproducibility for advanced tissue models.-Specialized in 3D organoid cell culture, establishing protocols for the growth, maintenance, and analysis of complex organoid systems for preclinical applications.-Identified… Show more -Optimized genetic manipulation of 3D organoids, enhancing transfection and gene editing efficiency to facilitate functional studies in complex tissue models.-Designed and implemented automated 3D organoid cell passaging systems, optimizing workflows and increasing reproducibility for advanced tissue models.-Specialized in 3D organoid cell culture, establishing protocols for the growth, maintenance, and analysis of complex organoid systems for preclinical applications.-Identified and developed innovative cell culture technologies to meet evolving needs in advanced cell culture systems for the Life Science community.-Advanced and implemented preclinical models focusing on liver, small intestine, kidney, and brain tissues to enhance drug safety assessment and target biology understanding.-Collaborated with cross-functional teams and internal partners in an international, cross-sectoral project setting, providing expertise in advanced tissue culture models.-Specialized in iPSC-derived organoid development, working closely with Merck Healthcare colleagues to enhance organoid systems.-Extensive cell culture expertise and academic networking, leveraging strong connections within the academic sector to support project needs and laboratory advancements. Show less -
Assistant ProfessorSanta Monica College Jan 2019 - Dec 2024Part-time 10+ hours-Supervised lecture and laboratory Bio3 evening classes. Taught the following topics: basic molecular and cellular biology, genetics, the anatomy and physiology of plants, animals, and humans, the diversity of life, evolution, and ecology -Communicated current environmental issues and new developments in the biological sciences -Laboratory experiences are integrated with lectures and stress scientific methodology and thinking -
Staff ScientistTeknova Mar 2022 - Feb 2024Full-time 40+ hours-Led CRISPR/Cas9 initiatives through cell line development that increased crude and purified Adeno-Associated Virus (AAV) full-to-empty ratios in cellular extract -Led Flow Cytometry assay development to test for AAV mammalian infectivity and mammalian transfection buffer assay development-Led mammalian cell culture laboratory to maintain cell lines for flow cytometry assays, mammalian transfection assay development, and AAV buffer development-Oversaw daily… Show more Full-time 40+ hours-Led CRISPR/Cas9 initiatives through cell line development that increased crude and purified Adeno-Associated Virus (AAV) full-to-empty ratios in cellular extract -Led Flow Cytometry assay development to test for AAV mammalian infectivity and mammalian transfection buffer assay development-Led mammalian cell culture laboratory to maintain cell lines for flow cytometry assays, mammalian transfection assay development, and AAV buffer development-Oversaw daily operations of the flow cytometry and cell culture laboratories, including maintenance, inventory, and user communications-Managed upstream and downstream AAV production and testing-ddPCR assay development -Generated 15+ unique cell lines through gene manipulation-Prepared white papers and facilitated protocol transfers to contract research organization-Managed two research associates-Met and/or exceeded all project goals and performance reviews-Played a crucial role in the launch of over 60 products dealing with AAV process development buffer and ddPCR assay buffers -Kept abreast of current biomedical science in the literature related to the functions of the current or future project. Recommended appropriate course of action to Research and Development Director, as deemed necessary Show less -
Crispr/Functional Genomics ManagerCalifornia Nanosystems Institute At Ucla Jan 2020 - Feb 2022Full-time 40+ hours-Developed, optimized, and implemented CRISPR/Cas9 technology for gene manipulation-Designed and optimized guide-RNA for CRISPR, maintained CRISPR libraries, proficient in next-generation sequencing for CRISPR pooled libraries-Led all lentiviral and protein production for core laboratory users and/or projects -Trained in primary cell immortalization, high throughput screening, and automated liquid handling -Proficient in recombinant DNA and bacterial… Show more Full-time 40+ hours-Developed, optimized, and implemented CRISPR/Cas9 technology for gene manipulation-Designed and optimized guide-RNA for CRISPR, maintained CRISPR libraries, proficient in next-generation sequencing for CRISPR pooled libraries-Led all lentiviral and protein production for core laboratory users and/or projects -Trained in primary cell immortalization, high throughput screening, and automated liquid handling -Proficient in recombinant DNA and bacterial cloning and compound library management-Managed Flow Cytometry instrument (BD Aira Fusion III) data acquisition, data analysis, instrument maintenance, and user communication-Managed high-throughput screening data acquisition and transfer-Generated over 300 cell lines with the use of CRISPR and lentiviral technology-Drafted, refined, and implemented standard operating procedures (SOPs)-Consistently met and/or exceeded all project goals and performance reviews-Kept abreast of current biomedical science in the literature related to the functions of the current or future project-Published three scientific papers with authorship Show less -
Staff Research Associate IiCalifornia Nanosystems Institute At Ucla Jun 2018 - Dec 2019High Throughput Screening (HTS), Automated Liquid Handling,Large Particle Flow Cytometry, Compound library management, Student assistant training and supervision, User / Client / Assay based specialized service, SOP creation, refinement, and implementation,Data acquisition, management, and analysis -
Post Doctoral Research FellowCharles R. Drew University Of Medicine And Science Apr 2016 - Jun 2018Los Angeles, CaFull-time 40+ hours Developed novel drug targets in Triple Negative Breast Cancer (TNBC) health disparities Created novel TNBC cell lines using RNAi, CRISPR, and lentiviral transduction methods Supervised and managed flow cytometry instruments (AttuneNxT flow cytometer, BD FACSMelody cell sorter) and Leica confocal microscopy operations Published two first-author scientific papers
David Austin Skills
David Austin Education Details
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Cancer Biology -
Biology -
Biology
Frequently Asked Questions about David Austin
What company does David Austin work for?
David Austin works for Milliporesigma
What is David Austin's role at the current company?
David Austin's current role is Principal, Staff Scientist | Experimental Design | Assay Development | Project Management | Research & Development | Microscopy | Flow Cytometry | SOP Generation | Cross-Functional Collaboration | Problem Solving | Piano.
What is David Austin's email address?
David Austin's email address is da****@****ilt.edu
What is David Austin's direct phone number?
David Austin's direct phone number is +121693*****
What schools did David Austin attend?
David Austin attended Vanderbilt University, Tuskegee University, Tuskegee University.
What skills is David Austin known for?
David Austin has skills like Leadership, Team Building, Powerpoint, Public Speaking, Biology, Western Blotting, Chemistry, Teaching, Matlab, Event Planning, Data Analysis, Clinical Research.
Who are David Austin's colleagues?
David Austin's colleagues are Courtney Webb, Dennis Bartlett, Paige Faircloth Vohlken, Robert Anderson, Catherine Waluck, Tani Leigh, Kissanet Birhane.
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Pacific Grove, Ca5yahoo.com, gmail.com, gmail.com, visa.com, dole.com5 +141529XXXXX
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Moraga, Ca4salesforce.com, salesforce.com, mymyopia.net, veza.com2 (800) 6XXXXXXX
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David Austin
Phoenix, Az4intel.com, intel.com, intel.com, nvidia.com -
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Dallas-Fort Worth Metroplex4ecisolutions.com, edadata.com, randallreilly.com, corcentric.com3 +181737XXXXX
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David Austin
United States2pwc.com, us.pwc.com
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