Nicholas Smith Email & Phone Number

Rensselaer, Ny, Us

Syracuse, New York, United States

Using single-cell RNA sequencing and analysis in R to understand human cytomegalovirus infection of primary monocyte subsets.Development and optimization of a primary monocyte model of HCMV latency and viral reactivation to understand HCMV latency maintenance by flow cytometry, western blotting, and other techniques.

Boston, Massachusetts, United States

Used CRISPR/CAS9 screen and TMT proteomics data to determine gene targets regulating Epstein-Barr virus latency and CRISPR / CAS9, flow cytometry, and western blotting to determine the effect on viral gene expression.

Aurora, Colorado

Dissertation Title: Epstein-Barr virus gp350 role in T-cell tropism and protection against infection: Implications for an EBV vaccineIdentified CD21 as the binding receptor for Epstein-Barr virus infection of primary human T-cells through neutralization assays, flow cytometry, real-time PCR, and CRISPR/CAS9 gene editing.Described a correlation between Epstein-Barr virus type 1 and 2 co-infection and diminished anti-gp350 IgA antibody response in a Kenyan infant cohort using a novel qPCR assay, ELISA, and Luminex.

Syracuse, New York Area

Identified CD21 as the binding receptor for Epstein-Barr virus infection of primary human T-cells through neutralization assays, flow cytometry, real-time PCR, and CRISPR/CAS9 gene editing.Described a correlation between Epstein-Barr virus type 1 and 2 co-infection and diminished anti-gp350 IgA antibody response in a Kenyan infant cohort using a novel qPCR assay, ELISA, and Luminex.Relocated with PhD mentor and transferred PhD studies to University of Colorado Anschutz Medical Campus.

Albany, Ny

Integral part of a team performing testing of clinical samples for a panel of viruses using real-time and conventional PCR and developing novel real-time RT-PCR assays for clinical approval for Powassan/Deer Tick virus, Dengue virus serotypes 1-4, and Chikungunya virus.

Burlington, Vermont

Studied the genetic variation within a population of Triatoma dimidiata, the primary insect vector of Chagas disease in Central America, with conventional PCR and DNA sequencing analysis.

Albany, New York

Doctor Of Philosophy - Phd, Immunology

Ba, Biology

Activities and Societies: UVM Club Football UVM Pre-med ClubMinor - Chemistry, Anthropology