Samuel Snyder Email & Phone Number

My expertise is in the field of protein chemistry. I graduated with a PhD in Chemistry from Saint Louis University in March 2023, after which I accepted a postdoctoral position at Argonne National Laboratory in April 2023. At Argonne, I am part of the Solar Energy Conversion Group where I work in a collaborative called the Center for Catalysis in Biomimetic Confinement (CCBC), an Energy Frontier Research Center of the U.S. Department of Energy. The CCBC focuses on gaining a fundamental understanding of bacterial microcompartments comprised of protein-based shells and their ability to optimize multi-step reaction pathways for confined cargo molecules and enzymes. My work involves expressing, purifying, and assembling these protein shells and subunits, which are then characterized upon inserting cargo.In my dissertation research, I used resonance Raman spectroscopy to study heme proteins from Mycobacterium tuberculosis, namely a noncanonical heme oxygenase called MhuD and a cytochrome P450 enzyme, CYP121. I used rR spectroscopy to investigate active site architecture affecting the heme environments of these proteins in various oxidation, spin and ligation states.I have acquired a number of technical skills throughout my research experiences. I have expertise in resonance Raman and UV-visible absorption spectroscopies. Additionally, I am proficient in protein expression, purification and cell culture work. I also have great familiarity with a variety of biochemistry techniques such as SDS-PAGE, agarose gel electrophoresis of nucleic acids, column chromatography, Bradford assays, pyridine hemochrome assays, and enzymatic activity assays. There are several computer softwares for data processing and graphic design that I am competent with including GRAMS, Image Lab, and Adobe Illustrator.